From: Albert Tai (acktai@exelixis.com)
Date: Wed Aug 27 2003 - 15:24:14 EST
Dr. Shlomchik, I have used the Annexin V detection kit from BD for early apoptosis detection and it worked well in our hands. The detection is based on FITC-Annexin V binding to phosphatidylserine translocated to outer membrane during early stage of apoptosis. Regards, Albert Tai Exelixis, Inc. South San Francisco, CA ----- Original Message ----- From: "Mark Shlomchik" <mark.shlomchik@yale.edu> To: cyto-inbox Sent: Tuesday, August 26, 2003 11:39 AM Subject: CASP-Glow Red > Hi All, > > I am wondering if anyone has experience with CASP-Glow Red from > Biovision. We have used CASP-Glow Green before successfully, but now > we want to combine this analysis with CFSE to measure apoptosis in > the labeled cells. The company says that they have used > sulforhodamine "beta", and that this can be used in the PE channel. I > have my doubts about this because from what I can find of the ex/em > profile of sulforhodamine (actually sulforhodamine 101 on > probes.com), it would seem to be very poorly excited by the 488 line > on the FACScalibur (the instrument we are using). > > However, it may be possible that it gives enough signal in practice > and so I wondered if anyone has tried it. I am also looking for > suggestions of measuring early and late apoptotic events using the > FL2 channel. > > Thanks, > > Mark > -- > Mark Shlomchik, MD, PhD > Associate Professor of Laboratory Medicine > and Immunobiology > Yale University School of Medicine > > 203-737-2089 > 203-785-5415 (fax) > mark.shlomchik@yale.edu > > OFFICE ADDRESS FOR FEDEX > > Yale University School of Medicine > 1 Gilbert St. > CAB S541 > New Haven, CT 06510 > > ADDRESS FOR POSTAL LETTERS (can take two weeks to reach me) > > Yale University School of Medicine > 333 Cedar St. Box 208035 > New Haven, CT 06520-8035
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:43:52 EST
