lacZ B-Galactosidase by flow

From: Lamb, Holly B. (lamb.holly@MAYO.EDU)
Date: Mon Aug 25 2003 - 16:14:56 EST

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Fellow Flowlanders,

We have a customer that is interested in using a lacZ B-Galactosidase detection kit on
our LSRII, which is currently equipped with the 325nm HeCd laser.  The information we
have found indicates that it is optimally excited around 390nm but we have been unable to
locate a spectral graph of the excitation of this substrate.

Does anyone have experience exciting CUG (3-carboxyumbelliferyI B-D-galactopyranoside)
with this laser line?  We would appreciate any suggestions or hints that you can offer.

Thanks for your help,

Holly


Holly Lamb
Flow Cytometry/Optical Morphology Research Core Facility
Mayo Clinic
Rochester, MN

lamb.holly@mayo.edu
507-284-4241

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