From: sstouden@thelinks.com
Date: Sun Aug 17 2003 - 01:53:55 EST
any biological reason why Mosquito viable cells would take up PI ? On Tue, 5 Aug 2003 ann.atzberger@talk21.com wrote: > > you can sort them much the same as any other cell line. I only use a viablity marker > where there is a high number of dead cells, otherwise discriminate on FSC.SSC... but you > can definitely use PI and very likely 7-AAD- same as mammalian cell lines. > Only weird thing I've come across was with Mosquito cells, viable cells also took up PI, > these were sorted (had too, they were 100% pos)proved not to be dead cells as they grew > in culture. > > regards > Ann Atberger > > > > -------------------- > talk21 your FREE portable and private address on the net at http://www.talk21.com
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