From: Alexander Schmitz (axs@mb.au.dk)
Date: Tue Aug 12 2003 - 04:21:18 EST
Dear Flowers, I am in the process to decide the configuration for a new Flowanalyser and/or FlowSorter. My general question is, how far the Violet Diode is able to substitute a watercooled UV laser, respectively which kind of applications really need a UV source ? For example DakoCyt sells the Cyan optional with a UV laser or a Violet Diode 405. Our facility is located within a basic research area, meaning that all kinds of "UV-related" applications could be on the task list. Some applications are: Sidepopulation sorting, Cell-cycle, Calcium-flux studies; Indo dyes, DAPI, etc. How much is it dependent on the laser, and how much is it dependent on the optics ? (I read the discussion in this forum concerning SP sorting using the Diode Laser on the FACSAria). Any comments are welcome Many thanks in advance ______________________________________ Alexander Schmitz Department for Molecular Biology (MBI) C.F. Moellers Allee 130/3 DK 8000 Århus Denmark axs@mb.au.dk
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