[7-AAD non-specific binding?]

From: Kristie Melnik (kmelnik@imeddinc.com)
Date: Thu Jun 19 2003 - 14:41:31 EST

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Has anyone ever had experience with 7-AAD nonspecifically labeling viable cells?  I had cell sample preps analyzed yesterday on a FACSCalibur and everything looked dead.  When I ran the same samples with propidium iodide, they looked fine.	I also did a trypan blue exclusion just to verify the viability of the cells.  Both the PI and the trypan blue had similar results.  The funny thing is I used 7-AAD last week on the same type of experiment and it worked perfectly.  Anyone have any thoughts??

Thanks,
Kristie


Kristie M. Melnik
Associate Scientist
iMEDD Inc.
1381 Kinnear Rd, Suite 111
Columbus, OH 43212


Phone --614-340-6001
FAX-- 614-340-6005

kmelnik@imeddinc.com

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