[Macrophage phagocytosis]

From: Veerupaxagouda (veeru@biochem.iisc.ernet.in)
Date: Wed Apr 23 2003 - 01:31:03 EST

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Dear flowers,

I am a graduate student working on macrophages...i am standardizing
phagocytosis assay using FITC tagged latex beads using flow cytometry....
i am facing following problems:

1.what is the best way to differentiate cells from the beads(that are non
specifically sticking to the cells inspite of frequent washings I do)?

2. what is the best way to represent the data?..right now I am subtracting
the negative control (the 4 degree negative control) histograms from the
test histograms....

thanks in advance...
bye
veeru  

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