From: Albert Tai (acktai@exelixis.com)
Date: Wed Apr 16 2003 - 12:23:30 EST
Hi Cheryl, Apparently Biostatus from UK sells a DNA dye for live cell staining which should be excited by 488 called DRAQ5 but I only have limited success with it, although I heard you may need to do extension titration to determine the optimal conc. Hope this help. Albert ----- Original Message ----- From: "Cheryl Smith" <cheryla.smith@utoronto.ca> To: cyto-inbox Sent: Wednesday, April 16, 2003 5:01 AM Subject: DNA dyes for viable cells > > Sorry people......I should have been more specific in my previous posting > (which I have copied here). I want to be able to sort by cell cycle G1 and > G2 populations. I'm not sure if the SYTO dyes will give this sort of > discrimination. > > Cheryl > > Hi Folks, > I can't think of any DNA dyes that would be excitable by 488 or 633 that > will stain viable cells. Are there any out there? > > Alternatively, has anyone tried transiently permeabilizing them by > electroporation and using PI? > > Thanks, > Cheryl >
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