From: Albert Tai (acktai@exelixis.com)
Date: Mon Mar 31 2003 - 14:52:37 EST
----- Original Message ----- From: "Albert Tai" <acktai@exelixis.com> To: cyto-inbox Sent: Monday, March 31, 2003 11:52 AM Subject: Re: cell cycle of endothelial cells > Hi Martin, > > Have you looked at your histograms under FL-2 ? Also treating your fixed > cells with RNase may improve the resolution of your histogram. I usually > incubate my cells in mixture of RNase and PI. > > Good luck, > Albert > ----- Original Message ----- > From: <Martin.Kjerrulf@astrazeneca.com> > To: "Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu> > Sent: Monday, March 31, 2003 6:29 AM > Subject: cell cycle of endothelial cells > > > > > > Dear All, > > > > I'm trying to do cell cycle analysis of human arterial endothelial cells. > > After detaching the cells with trypsin and fixing in 70% ethanol I stain > > them with propidium iodide. > > I acquire the cells in the Calibur using linear FL-3 scale and analyse the > > data using Winlist software. > > My problem is that the G1 and G2 peaks come very close to each other. The > > S-phase is barely visible in the diagram. > > Does anyone know how to get the G1 and G2 peaks more separated? > > > > Martin Kjerrulf > > AstraZeneca R&D, > > Molndal, Sweden > > > > >
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