FITC labelled bacteria

From: Analía Rial (arial@higiene.edu.uy)
Date: Fri Mar 21 2003 - 07:31:11 EST

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Hi. I would like to post a non flow cytometry question. Neverthless, I
hope someone out there can help me.  The thing is I am trying to label
inactivated bacteria with FITC, to use in an intranasal murine model ( to
further analyze lung DC by FC). I do have a protocol to do this, but I
wanted to know if is it possible to label the heatinactivated bacteria and
then storage them (i.e. at -80ºC) in order to use them for the
immunization protocol afterwards, or if, on the other hand it is esential
to labell and use them immediately. I will appreciate any help you can
provide. Thanks in advance
Analía Rial
Lab. for Vaccine Research
Montevideo, Uruguay

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