From: David Coder (d_coder@MSN.com)
Date: Thu Feb 27 2003 - 12:29:49 EST
This brings up the issue of validating procedures--something that almost no one does unless required to as fundamental part of running a clinical or manufacturing laboratory. So, I'd agree: unless you have demonstrated (and do so on a periodic basis) that your methods and materials consistently give the results that you claim, you may just be whistling in the dark. Dave ====================== David M. Coder, Ph.D. Consultant in Cytometry Seattle, Washington tel./message: 206-499-3446 email: d_coder@msn.com -----Original Message----- From: Howard Shapiro [mailto:hms@shapirolab.com] Sent: Tuesday, February 25, 2003 10:35 PM To: cyto-inbox Subject: RE: Sorting live human lymphocytes A question that I will ask because it has not come up on this thread is: What about "fixed" lymphocytes? My recollection is that 1) the papers about HIV inactivation by various fixatives are pretty old, and 2) people who fix lymphocytes use fixatives that are reported to inactivate HIV, but I wouldn't take bets on whether the fixative concentrations and fixation times are greater than or equal to those reported to be necessary to completely inactivate virus in an infected specimen (which would presumably vary with viral load). Just thought I'd ask. -Howard
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