From: Andrea Cumming (a.cumming@niwa.co.nz)
Date: Wed Feb 26 2003 - 15:27:25 EST
Hello all We recently had a few problems with our Facscalibur while out at sea. The first problem was with the flow rates, we had to run all of our samples on high flow, as medium flow was only working intermittently (when running a sample the event rate would vary and sometimes drop to 0 and stop.) Low flow wasn't working at all, we would get 0 events/second and when the same sample was run on the same settings on high flow we would get 2000 events/second!! Secondly when viewing a forward vs side scatter dot plot the events would move up and down on forward scatter in time with the movement of the ship (good to watch if you want to get seasick). Apparently these problems were caused by the movement of the ship creating back pressure in the waste line, however it didn't seem to improve when we ran a waste line directly from the flow cell to a bucket on the floor (no back pressure). We have taken this machine out to sea before and not seen these problems. Has anyone else had similar problems or have any ideas on what we can do to eliminate it? Thank you Andrea _______________ Andrea Cumming NIWA (National Institute of Water & Atmospheric Research) Gate 10, Silverdale Road, Hamilton, New Zealand Phone +64-7-8567026 DDI +64-7-8591856 Fax +64-7-8560151
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:43:33 EST
