From: Kenneth Margulies, MD (margul@unix.temple.edu)
Date: Wed Feb 26 2003 - 06:37:40 EST
I would like some input regarding investigators' experience with using flow cytometry to sort isolated cardiac myocytes by size. These rod-shaped cells range in size from 1,000 to 100,000 cubic microns with quite a lot of variability even within a normal heart. Typical dimensions are: 120 microns long by 18 microns wide by 10 microns thick. My goal would be to sort the cells into broad size ranges (e.g. quartiles). Has anyone done this? What sized aperture would be needed?, etc... Thanks, Ken Margulies, M.D. Temple University
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