From: McCoy, J. Philip (NIH/NHLBI) (McCoyJ@nhlbi.nih.gov)
Date: Fri Feb 21 2003 - 16:07:36 EST
This post got me thinking a bit about this topic. Does anyone have any first hand knowledge of an operator being infected from doing a sort of human material? While I certainly advise universal precautions when handling human specimens, I am not aware of any documented sort-related infections in my 25 years of doing flow. If there are some, I think it would make an interesting, and much needed, CDC report. Phil -----Original Message----- From: J.Paul Robinson To: cyto-inbox Sent: 2/19/2003 9:04 PM Subject: Sorting live human lymphocytes Colleagues: I would like to get input into the following issue - this has been discussed before, but I would like to put this topic into the summary page and I also need some advice. There is a faculty member here who is insisting on sorting live human lymphocytes from untested patients. His argument is that these are from children or teenagers and therefore a low-risk group. He obtains the mateirals from a clinic and claims that he has no time to test the samples. He is unbelievably insistent (my techs say he is rude and obnoxious) and is very upset that I have told him that I need some time to research this issue to see what we should do. Even after I stopped a sort from taking place instructing my technicans not to sort the cells, he tried to convince them to sort after I left for a meeting!! He claims that he has done dozens of similar live human sorts at several major institutions (I am checking so I won't list the institutions here!) He claims that "many of the major papers in the immunology literature sort live human lymphocytes, so why can't you do that here? Other institutions do it all the time...." Has anyone actually tracked the number of such sorts? So my questions are the following: 1. What is your institution/lab policy on sorting live human materials? 2. Does your institution list this policy on a web site 3. How many of these sorts do you do? 4. Do any of you have obnoxious faculty that treat your techs like dirt? If not, we have one you can have! I will be happy to sumarize the discussion and post it to the new summary page at http://www.cyto.purdue.edu/hmarchiv/cytomail.htm "view Summaries" link Regards Paul Robinson Purdue J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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