From: Inna.Cohen@med.va.gov
Date: Fri Feb 21 2003 - 08:56:14 EST
Dear Flow-ers, We have a problem. We are trying to analyze HPAEC's(Human Pulmonary Endothelial Cells) using flow cytometry. We are looking at cell cycle distribution after hypoxia. Our problem is that most of our cells are destroyed before we can analyze them. We batch them in ethanol and then use the high salt extraction method but most of the cells are simply blown away by the time we analyze them. So we tried using the Cell Cycle kit from B-D which uses detergent to break open the cells but we get the same results. The cells just seem to burst before we can analyze them. Does anyone have any suggestions? If anyone else out there in flow land is working with these kinds of cells, any input would be greatly appreciated. Thank you, Inna
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