From: Fischer, Randy (NIH/NIAMS) (fischer1@mail.nih.gov)
Date: Thu Feb 20 2003 - 14:22:09 EST
Paul, We do sort live human lymphocytes. Conditions- pre-tested for HIV, HepA-C, and anything else the clinicians think would be dangerous to us. Also, we have aerosol containment on our sorter. So far, no problems. I would suggest you tell your "client" to take his business elsewhere, not because you can't technically sort his specimens, but because he has not met certain criteria you need for the safety of your staff. And even were he to say he would test the donors, I would be very hesitant to believe someone who has a history like his. Randy T. Fischer NIH/NIAMS Building 10, Room 6D50 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: J.Paul Robinson > Reply To: jpr@flowcyt.cyto.purdue.edu > Sent: Wednesday, February 19, 2003 9:04 PM > To: Cytometry Mailing List > Subject: Sorting live human lymphocytes > > > Colleagues: > > I would like to get input into the following issue - this has been > discussed > before, but I would like to put this topic into the summary page and I > also > need some advice. > > There is a faculty member here who is insisting on sorting live human > lymphocytes from untested patients. His argument is that these are from > children or teenagers and therefore a low-risk group. He obtains the > mateirals > from a clinic and claims that he has no time to test the samples. > > He is unbelievably insistent (my techs say he is rude and obnoxious) and > is > very upset that I have told him that I need some time to research this > issue to > see what we should do. Even after I stopped a sort from taking place > instructing my technicans not to sort the cells, he tried to convince them > to > sort after I left for a meeting!! > > He claims that he has done dozens of similar live human sorts at several > major institutions (I am checking so I won't list the institutions here!) > > He claims that "many of the major papers in the immunology literature sort > live > human lymphocytes, so why can't you do that here? Other institutions do it > all > the time...." > > Has anyone actually tracked the number of such sorts? > > So my questions are the following: > > 1. What is your institution/lab policy on sorting live human materials? > 2. Does your institution list this policy on a web site > 3. How many of these sorts do you do? > 4. Do any of you have obnoxious faculty that treat your techs like dirt? > If not, > we have one you can have! > > I will be happy to sumarize the discussion and post it to the new summary > page at > http://www.cyto.purdue.edu/hmarchiv/cytomail.htm > "view Summaries" link > > Regards > Paul Robinson > Purdue > > J.Paul Robinson, PhD PH:(765)4940757 > Professor of Immunopharmacology > Professor of Biomedical Engineering > Purdue University FAX:(765)4940517 > EMAIL:jpr@flowcyt.cyto.purdue.edu > WEB: http://www.cyto.purdue.edu >
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