From: Hodge, Greg (HAEM) (hodgeg@mail.wch.sa.gov.au)
Date: Wed Feb 19 2003 - 00:19:36 EST
> -----Original Message----- > From: Mihaela Roxana Cimpan [SMTP:mihaela-roxana.cimpan@cih.uib.no] > Sent: Tuesday, 18 February 2003 8:41 PM > To: Cytometry Mailing List > Subject: Fwd: [Plant cell suspension prep] > Importance: High > > Mihaela, > we have sorted apoptotic lung epithelial cells stained with Annexin V > FITC which is available through several companies (we use Pharmingen). > Follow their suggested protocol for staining which is basically wash cells > in a Ca++/Mg++ buffer, add 2 uL of Annexin V FITC (in buffer) to cells > then sort Annexin V pos cells. Following sorting and checking for purity > one can simply remove the Annexin by washing cells in Ca++/Mg++ free > buffer. If you only want early apoptotic cells you will also need to stain > cells with PI or 7-AAD and sort Ann+/PI- cells. > Hope this helps. > > Greg Hodge PhD > Senior Medical Scientist, > Flow Cytometry Laboratory > Haematology Department, > Women's and Children's Hospital > 72 King William Rd, > North Adelaide, > South Australia 5006 > ph 08 81616089 > fax 08 81616043 > hodgeg@mail.wch.sa.gov.au > > > >Dear all, > > > >Would anybody kindly suggest a protocol for sorting apoptotic cells in > >suspension? I would like to remove the apoptotic cells. > > > >Your halpfulness is already appreciated, > > > Mihaela Cimpan
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