Re: Is DRAQ5 Fluorescence Affected by Bromodeoxyuridine?

From: Albert Tai (acktai@exelixis.com)
Date: Fri Feb 07 2003 - 18:22:25 EST

Next message: randy huang: "Coulter Sorter for sale!"
Previous message: Tara McDonald: "CFP and GFP... The Summary"
In reply to: Howard Shapiro: "Is DRAQ5 Fluorescence Affected by Bromodeoxyuridine?"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

Dr. Shapiro,

I have used DRAQ5 in DNA staining in live cells and got reasonble profile.
However the %CV went up to about 10 - 15% relative to 3 -  5% in the case of
methanol fixation with PI.  Could you recommend areas of optimization for
DRAQ5 staining ?  Any thoughts would be greatly appreciated.

Thanks,

Albert Tai
Exelixis, Inc.
South San Francisco.
----- Original Message -----
From: "Howard Shapiro" <hms@shapirolab.com>
To: cyto-inbox
Sent: Thursday, February 06, 2003 4:30 PM
Subject: Is DRAQ5 Fluorescence Affected by Bromodeoxyuridine?


>
> DRAQ5 and Hoechst 33342 appear to the only dyes that can reliably produce
> stoichiometric staining of DNA in live cells. Hoechst 33342 is, as most
> people know, quenched by BrUdR (or BrdU, if you prefer). If DRAQ5
> fluorescence is unaffected by BrUdR, or even, perhaps, if it is enhanced
by
> BrUdR,not, the combination of the two dyes might be usable to detect BrUdR
> incorporation in live cells, which would be of interest.  Has anybody
tried
> this?
>
> -Howard
>

Next message: randy huang: "Coulter Sorter for sale!"
Previous message: Tara McDonald: "CFP and GFP... The Summary"
In reply to: Howard Shapiro: "Is DRAQ5 Fluorescence Affected by Bromodeoxyuridine?"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:43:30 EST