From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Wed Jan 29 2003 - 22:46:02 EST
Hi all, We are seeing a lot of on-axis events on our FACSDiva. We have been led to believe that this is 'normal' and due, at least in part, to measuring area rather than height. We are wondering a couple of things... i) does anyone have a good (simple?) explanation of why it occurs - some of the users here are getting very disconcerted by it and would like to understand what is going on. ii) what is reasonable level of on-axis events? In some cases were are seeing more than 50% of the events piled up on the axis and fiddling with the PMTs doesn't seem to make much difference... Thanks, Adrian Smith -- ______________________________________________ Adrian Smith (Research Officer) T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. Ph: (02) 9565-6198 Fax: (02)-9565-6103
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