From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Tue Jan 28 2003 - 12:44:12 EST
As an addition to my comments about replies I received concerning looking at DsRed with 488nm excitation: There is a difference between different forms of DsRed. Consensus is that DsRed (or DsRed1) is weak and/or useless --- but that DsRed2 or DsRed3 are much better. The most detailed reply I received recommends DsRed Express (also called DsRed.T1) from Clontech. Again, most replies were very positive about exciting DsRed (one form or another) with a 488nm laser. Some replies mentioned doing this on a FACSCalibur. Most other replies did not specify cytometer (or indicated that it was a sorter). Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
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