From: Albert Tai (acktai@exelixis.com)
Date: Wed Jan 15 2003 - 12:06:00 EST
----- Original Message ----- From: Hyun, Bill To: cyto-inbox Sent: Monday, January 13, 2003 8:24 PM Subject: RE: doublet discrimination Hello Albert, We can do so on "model" doublet systems, like budding yeast or bead clumps...but the only way to be sure is by selective gating and sorting to delineate the cutoff on FSC and SSC for the doublets. All the best, -Bill ********************************** William C. Hyun, Director Laboratory for Cell Analysis, Room 129 UCSF Comprehensive Cancer Center San Francisco, CA 94143-0808 Phone: 415-476-2632 FAX: 415-476-8218 email: hyun@cc.ucsf.edu Federal Express and Delivery Address: 2340 Sutter Street, Room S-129 San Francisco, CA 94115 Mailing Address: 2340 Sutter Street, Box 0808 San Francisco, CA 94143-0808 This message is intended solely for the individual(s) and entity(s) addressed. It is confidential and may contain legally privileged information. The use, copying or distribution of this message or any information it contains, by anyone other than the addressee, is prohibited. If you have received this message in error, please notify hyun@cc.ucsf.edu. The mailbox address from which this message has been sent is for business mail only. Mail sent to it may be subject to security scanning and delivery of non-business messages sent to this address may not occur. Thank you. -----Original Message----- From: Albert Tai [mailto:acktai@exelixis.com] Sent: Friday, January 10, 2003 6:11 PM To: Cytometry Mailing List Subject: doublet discrimination Hi fellow flow-ers, I am wondering if there is a way to identify doublet vs single cell using FSC vs. SSC as a criteria rather than doing pulse/width analysis ? Any input will be much appreciated. Thanks, Albert Tai Exelixis Inc. Signal Transdcution Research Group.
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