Sorting CHO cells?

From: PAUL HALLBERG (Paul.Hallberg@mail.tju.edu)
Date: Wed Dec 11 2002 - 16:38:48 EST

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I would appreciated any suggestions to facilitate sorting CHO cells which
express VE-cadherin. These cells tend to aggregate making sorting difficult.
Vortexing seems to increase aggregation. These adherent cells are removed
from the culture flask using trypsin("Cell Stripper"). I try to keep the
cell concentration low which helps somewhat, but is not ideal. Is any buffer
available that will not destroy VE-cadherin?

Thanks in advance,


Paul L. Hallberg
Flow Cytometry Manager
KCC CORE Flow Cytometry Facility
Thomas Jefferson University
215-503-4556
215-923-0249(fax)
Paul.Hallberg@mail.tju.edu

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