Re: fix first stain later for surface antigens

From: Howard Shapiro (hms@shapirolab.com)
Date: Tue Nov 26 2002 - 16:50:03 EST

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Justin Fishbaugh wrote-

>We have an investigator that needs the ability to fix human blood and stain
>later for surface and possibly intracellular antigens.   A search of Medline
>came up with Jani et al, "Affordable CD4+ T cell counts by flow cytometry
>II. The use of fixed whole blood in resource-poor settings," Journal of
>Immunological Methods, Volume 257, Issues 1-2, Pages 145-154.  This article
>talks about using a fixative called TransFix made by UK NEQAS before
>staining.   A search of the UK NEQAS website did not find anything
>referencing TransFix.  Does anyone have experience with this stuff or have
>some other magic potion that would do the trick?  Thanks.

David Barnett <d.barnett@sheffield.ac.uk> developed TransFix; if there
isn't anything about it on the NEQAS site, he should be able to provide
details.

-Howard

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