From: Jayaraman, Sundararajan (SJayaram@med.miami.edu)
Date: Thu Nov 21 2002 - 15:08:18 EST
I have not used the BD kit for measuring apoptosis. However, note that MCF-7 cells lack functional caspase-3 owing to the deletion of the caspase-3 gene. Therefore, this kit will not be suitable for use in MCF-7 cells. There are a number of other alternative methods one can use to assess apoptosis. Our recent data indicate that caspase-7 is cleaved during apoptosis in MCF-7 cells. Jay > ---------- > From: Hill, Ron [R&D/0111] > Sent: Wednesday, November 20, 2002 7:15 PM > To: Cytometry Mailing List > Subject: Caspase Measurement > > > Hi all, > Has anyone had success with measuring apoptosis using BD's kit for > activated > caspase 3? We are trying to use it with MCF-7 cells. Are there any tricks > required for using this kit on adherent cells? > Thanks in advance > Ron Hill > > Ronald J. Hill, Ph.D. > Sr. Group Leader, Research > Sugen, Inc. > 230 East Grand Ave. > South San Francisco, CA 94080 > ron-hill@sugen.com > tel: 650-837-3797 > fax: 650-837-3313 > >
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