From: Richard Haugland (richard.haugland@probes.com)
Date: Tue Nov 12 2002 - 09:23:52 EST
Does it have a dye on it? If it is labeled with most dyes (except phycobiliproteins) then trypan blue is an almost universal quencher of external antibodies (especially fluorescein and Alexa Fluor 488) but not internalized fluorescent antibodies. Useful trick for answering "inside-outside" questions. "Barren, Phil" wrote: > Could some one point me in the right direction? > > I have an investigator that wants to determine if their antibody is > internalized post binding to the surface of cells. > > Could someone offer some advice on detection, controls etc?? > > Thank you > > Philip Barren
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