From: Finney, Simon (s.finney@ic.ac.uk)
Date: Sat Nov 09 2002 - 06:47:47 EST
Dear All, As a rather minor part of someones project I would like to isolate a relatively pure preparation of rat granulocytes, stimulate them ex vivo, and then stain for surface markers/ F actin. We wanted to stimulate a pur(ish) population. I found a simple gradient system for purification called NIM2 from Cardinal Associates on the net but their telephone number is unobtainable - does any one know who supplies it nowadays or alternatively of a simple purification method. Thanks Simon ---------------------------------------------------------------------------- ----- Dr Simon Finney Clinical Research Fellow National Heart and Lung Institute Dovehouse Street London s.finney@ic.ac.uk
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