From: Eric Van Buren (aa9080@wayne.edu)
Date: Sun Nov 10 2002 - 14:11:21 EST
Dr. Barton, We've used Annexin V conjugated to phycoerythrin (PE), along with 7-amino- actinomycin D (7-AAD) as a viability marker. Both fluorochromes are com- patible with a 488 nm excitation source and EGFP. I believe we used the "Annexin V : PE Apoptosis Detection Kit I", Cat. No. 559762, from BD Pharmingen -- which includes Annexin V-PE, 7-AAD, and Annexin V binding buffer -- but these reagents may be available from other vendors. Good luck, Eric >Hello Flow-ers, > >I need a fluorochrome-conjugated to annexin V to check out my >transfectants, which have EGFP. I have only a 488 laser. What's the >best dye to use with EGFP transfectants? >Thanks in advance. > >Beverly > >Beverly E. Barton, Ph.D. >Assistant Professor >Department of Surgery > New Jersey Medical School > Box 1709 >185 S. Orange Ave. >Newark, NJ 07101 >phone 973-972-0662 >fax 973-972-6803 >e-mail bartonbe@umdnj.edu Eric Van Buren, eric.vanburen@wayne.edu Karmanos Cancer Institute and Immunology & Microbiology Wayne State University, Detroit, Michigan, USA
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:09 EST
