From: Simon Monard (smonard@trudeauinstitute.org)
Date: Thu Nov 07 2002 - 16:40:37 EST
Hi Allen
What sort of pathogens will you be dealing with?
I sort a wide variety of pathogens many of which are deadly...to our murine friends. My
lab is not negative pressure and I have a custom class I type biosafety cabinet mounted
to the sorter. I'd do it differently if I worked with human material or pathogens
dangerous to humans. The MoFlo Class I hood thing looks pretty good. Assuming human
material and HIV level stuff you would probably want to have the sorter in a dedicated
BL-3 room with restricted access with either a Class I hood type arrangement on the
instrument, a clean air mask on the operator or both.
Just my opinion
Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983
Ph 518 891 3080 X352
>>> allen parmelee <allen.parmelee@tufts.edu> - 11/5/02 5:56 PM >>>
Hi
we're looking into setting up a sorter facility that would handle a wide
variety of pathogens.
does any one know of any labs set up for this?
the only ones i'm aware of are only set up for 1 particular pathogen.
also, does any one have any recommendations for design considerations?
thanks for any help!
have a good one
allen
--
Tufts Laser Cytometry
Allen Parmelee Manager
617-636-0464 allen.parmelee@tufts.edu 617-636-2990 (fax)
a core facility offering:
MoFlo (Cytomation), FACSCalibur (Becton Dickinson) ,
LSC (CompuCyte), LCM (Arcturus)
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