From: Juergen Ostwald (juergen.ostwald@med.uni-rostock.de)
Date: Tue Sep 17 2002 - 04:16:22 EST
Dear flowers Please let me in with a question about cell culture, only touching the field of flowcytometry. I dissociate epithel-cells from nasal mucosa and culture these cells in collagencoated flask and petris with good success. But after the next dissociating step for splitting only a very small number of cells become adherence again to growth further. I have used trypsin/EDTA with subsequently treatment with FCS or Trypsininhibitor and also I have used Accutase-it's always the same bad result. Are there other enzymes or enzymecombinations or ready products which work better in may be very sensitive cells? Thank You for every answer. J.Ostwald ENT-Clinic University Rostock
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