From: Lee, Siow Fong (sflee@cha.ab.ca)
Date: Mon Aug 12 2002 - 13:22:59 EST
Hi All, I am posting the following questions for a colleague (cmattar@ualberta.ca) who uses a Beckman Coulter Ultra for sorting: When sorting an event from a post-nuclear cell supernatant, during the actual sort when the event is diverted into the sorting vessel, does some of the cell lysate that the event was suspended in originally also enter the sorting tube? Will soluble proteins floating around in the post-nuclear supernatant get sorted along with the event of interest? I realize the sample is taken up along with sheath fluid. Does this also dilute the non-specific soluble proteins which may contaminate a sorted sample? Thanks. Siow Fong Lee Department of Laboratory Medicine & Pathology University of Alberta Hospital Capital Health Authority Edmonton, Alberta Phone: (780)407-8067 Fax: (780)407-8599 E-mail: sflee@cha.ab.ca
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