From: PeterG (gogy@freemail.hu)
Date: Fri Jul 19 2002 - 06:40:32 EST
Hello Alfredo, It is depend on your cytometer. You should have UV laser. FURA-2 is used mainly for microscopy. Indo-1 is used in flow cytometry instead. If you have 488nm blue laser, you can use eg. Fluo-3 as Ca++ indicator. But in this case you cannot do ratiometric measurement directly. You can find the reasons and quiet good descriptions about different Ca++ indicators and methods here: http://www.probes.com/handbook/sections/2000.html Best regards, Gogy Peter Gogolak, Ph.D. Institute of Immunology University of Debrecen, Hungary Phone/Fax: +36 52 417159 Your original message: Dear flowers, I am interested in studying calcium response in human blood leukocytes after a specific stimulation. I used to analyse intracellular calcium in many cell types by a spectrofluorimetric method using FURA 2AM. May I use the same fluorochrome in flow cytometry? Do you have any suggestions on the matter? Any hint is welcome! Thank you in advance, Alfredo Vannacci ------------------------------------------------------------ ----------- Alfredo Vannacci, MD Department of Preclinical and Clinical Pharmacology Medical Toxicology Unit University of Florence Viale Gaetano Pieraccini, 6 Firenze 50139, Italy tel: +39(0)554271236 fax: +39(0)554271280 email: alfredo.vannacci@unifi.it
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