From: Nigel Blackhall (Nigel.Blackhall@nottingham.ac.uk)
Date: Thu Jul 11 2002 - 04:17:36 EST
Dear all Nigel Blackhall Senior Experimental Officer Plant Science Division School of Biosciences University of Nottingham Sutton Bonnington Campus Lougborough Leicestershire tel +44 (0) 115 9515151 ext 18501 fax +44 (0) 115 9516334 >>> Gisele Knowles <gknowles@sten.sunnybrook.utoronto.ca> 09/07/02 22:39:42 >>> Hello colleagues- Does anyone know of a product that can sit outside a cell and with either electroporation or other such stressor, enter the cell and then fluoresce?? I have this in my brain that such a thing exists and I'm concurrently trying to avoid using fitc dextran altogether because I'm finding too much non-specific fluorescence. Anyway all comments would be appreciated. Thanks May I suggest you look at the following article Flow cytometric quantification of electroporation-mediated uptake of macromolecules into plant protoplasts. Blackhall, N. W.; Finch, R. P.; Power, J. B.; Cocking, E. C.; Davey, M. R. Protoplasma 186 (1-2) 1995. 50-56. In this paper we looked at the non-specific fluorescence, and showed that it was caused by unbound FITC. The problem is considerably lessened by using purified FITC-dextran from Molecular Probes. Regards Nigel
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