Hi everyone,
I am working with frozen PBMCs and using CD27 in combination with CD45RA to define
naive cells (RA+27+). My idea is that CD27 can be used instead of CD62L to define these
cells, because expression of CD62L is lost in frozen cells. I assume this is based
in the fact that in certain subsets these two molecules are coexpressed in the same
cells, and in fact I have found that this is the case in the CD8 subset (all CD62L+
cells are CD27+). My question is: is this just a coincidence or is that CD27 function
is related to the naive phenotype?
Many thanks
José Miguel Benito
Hospital Carlos III
Madrid
