From: Howard Ratech (hratech@montefiore.org)
Date: Mon May 13 2002 - 13:46:03 EST
What cell line(s) do you recommend as a postive control? Sincerely, Howard Ratech, MD Howard Ratech, MD Director of Hematopathology Director of Molecular Pathology Lab Montefiore Medical Center Pathology - North 4 111 East 210th Street Bronx, NY 10467 Telephone: 718-920-7782 FAX: 718-920-7611 E-mail: ratech@aecom.yu.edu >>> ricardo morilla <ricardo@icr.ac.uk> 05/13/02 07:50AM >>> Dear All, The question of the detection of Cyclin D1 by flow has been discussed at length in this group during the past year. There are still people asking for a method to detect it. I enclose the method that we currently use in the lab and it is working in our hands. I hope that it will be of help to those of you who are trying to detect Cyclin D1 by flow. Ricardo. Reference: "Cyclin D1 by flow cytometry as a useful tool in the diagnosis of b-cell maliganacies" Manal O. Elnenaei et al. Leukaemia Research 25 (2001) 115-123 ========================================================== Mr. Ricardo Morilla Academic Department of Haematology & Cytogenetics The Royal Marsden Hospital 203 Fulham Road, London SW3 6JJ, UK Tel +44 (0) 20 7808 2882; Fax +44 (0) 20 7351 6420 ==========================================================
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