mouse B cells

From: Stetler-Stevenso, Maryalice (NCI) (stetler@mail.nih.gov)
Date: Thu Apr 25 2002 - 15:39:37 EST

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Here is a question for all of you mouse immunologists out there. The
immunophenotype of human memory B cells is CD20+, CD38-, CD44+ and CD86+. In
mice you need to use CD138 instead of CD38 to exclude plasma cells. If you
look at CD19 or B220+, CD138-, CD44+ and CD86+ cells in mouse spleen post
immunization do you detect memory B cells? Are plasma cells CD44+? Also-
does MUM-1 expression mean plasma cell differentiation or can this be
expressed in memory B cells or pre-plasma cells?


Maryalice Stetler-Stevenson, M.D., Ph.D.
Chief, Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH

Sometimes you're the windshield, sometimes you're the bug.

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