Some isotypes are stickier than others?

From: Karim Vermaelen (Karim.Vermaelen@rug.ac.be)
Date: Fri Apr 26 2002 - 10:13:49 EST

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Hello,
Does anyone know why some mouse Ab isotypes give more background than others
when staining human cells? We stained single cell suspensions form human
lung enzymatic digests and have a lot of problems with mouse IgG2a
antibodies: the isotype control stains very bright. Mouse IgG1 and IgG2b are
less of a problem...
Could someone explain this selective phenomenon and/or recommend an optimal
blocking strategy to get rid of the problem?
Thanks a lot,
Karim

• Next message: Guenter Valet: "2nd EWGCCA EuroConference, Urbino, Sep.19-22,2002"
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• Reply: Mike Clark: "Re: Some isotypes are stickier than others?"
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