Moving BrdU peak

From: Philippe Pognonec (Philippe.Pognonec@unice.fr)
Date: Wed Apr 10 2002 - 05:43:33 EST

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Hi there. Does anybody here has the answer to the following observation?
We do proliferation analysis on mouse thymocytes (individual cells, no clumps), using
BrdU labelling and a FACScan. We get on our
histograms a nice peak corresponding to the non proliferating cells, and a few percents
of cells proliferating, crawling out of the right
base of that peak. Fine.
The problem is that the large peak appears to be "moving" on the x axis from one sample
to the other, in a mysterious manner, without
any apparant correlation with animals, treatments, and so on. Something like 1 out of
5 measurements made the same day with the
same setting shows that weird phenomenon...
Any suggestion?
Philippe.

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