From: Howard T Petrie (h-petrie@ski.mskcc.org)
Date: Thu Mar 21 2002 - 17:21:32 EST
Well, unless you do a direct comparison (who's got that much antibody to waste?) it's hard to make more than an empirical judgement. I've made some dozens of A488 conjugates, and I would have to say that by flow, it's definitely brighter, but my estimate would be on the order of 2-4X. Using mercury excitation on a fluorescent microscope, however, it's quite substantially brighter than FITC. So overall, I would say the claims are reasonable. We like the Alexa series very much. I have no financial association with Molecular Probes (except that I buy their products. Lots of them.). HTP >On Molecular Probes' website are claims that Alexa 488 is 6-7 times brighter >than FITC. Preliminary evidence from our conjugations of the same antibody >with FITC or Alexa 488 indicate no advantage from using Alexa 488. Can anyone who compared Alexa 488 and FITC share their findings? -- Howard T. Petrie, Ph.D. Head, Laboratory of Developmental Immunology Memorial Sloan-Kettering Cancer Center Box 341, 1275 York Avenue New York, NY 10021 phone (212)639-2149 fax (212)794-4019
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