Vascular Smooth Muscle Cells

From: Kenichi Fujise (kenichi.fujise@uth.tmc.edu)
Date: Mon Mar 18 2002 - 20:28:19 EST

• Next message: Joseph Webster: "Re: 256 vs. 1024 channels"
• Previous message: Kraus, Elizabeth: "CELL DEATH"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

Hello!!

I am a cardiovascular investigator trying to isolate vascular smooth
muscle cells from rat carotid arteries at as high purity as possible.
Unfortunately, a previous attempt showed that only 25% of isolated
cells  were alpha-actin positive after differentiation (heparin and 0.5%
sera for 4 days).  Other cells appear to be endothelial cells.

Does anybody have a good protocol for vascular smooth muscle cell
isolation??
In addition, do you have any cleaver way to use FACS to determine the
purity of the prep?

I thank you for your time and help on this.

Sincerely,

Ken

• text/x-vcard attachment: Card for Ken Fujise

• Next message: Joseph Webster: "Re: 256 vs. 1024 channels"
• Previous message: Kraus, Elizabeth: "CELL DEATH"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:31 EST