From: Nadeem Sheikh (nadeem@bart.rprc.washington.edu)
Date: Thu Jan 31 2002 - 13:23:27 EST
Can anyone point me in the right direction for immunophenotyping cells from
cerebrospinal fluid. Specific questions I have are:
1. What kind of volume is usually taken
2. What are the normal population of cells - phenotype, nos. ?
3. Any safeguards/controls for blood contamination ?
I should point out that we are looking to establish techniques to look at
cellular infiltrates (if we can isolate any) in CSF of macaques infected
with SIV/SHIV that subsequently develop NeuroAIDS. Any advice, pointers to
papers would be most welcome human or macaque work.
With regards,
Nadeem
Nadeem Sheikh, PhD
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University of Washington
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