labeling of insect cells

From: Dominik Stoll (dominik.stoll@twinstrand.com)
Date: Thu Nov 22 2001 - 19:11:49 EST

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dear all,
We are trying to label insect cells, which are displaying a lectin on the
surface. We would like to use fluorescently labeled carbohydrates (e.g.
glycan of ASF) as a probe. Has anybody done FACS analyses with fluorescently
labeled carbohydrates (oligosaccharides)? I am worried that unspecific
binding will be a major problem.
I would appreciate any thoughts on that topic and recommendation on how to
label the oligosaccharides.
thank you very much.


Dominik Stoll

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