From: Ray Hicks (rh208@cam.ac.uk)
Date: Fri Sep 28 2001 - 12:41:24 EST
Hi Phil, Is this historic or fresh damage? If it's historic, looking for gross chromosomal abnormalities / partial aneuploidies might be a good approach (possibly easier with a microscope). If it's fresh, how about nick-translation with labelled bases, or for gross damage, DNA cell cycle perturbation? Ray > From: "Simon Monard" <smonard@trudeauinstitute.org> > Date: Thu, 27 Sep 2001 16:42:37 -0400 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Re: Any thoughts on the detection of Ionizing radiation effects in > rats > > > Hi > Could you look for chomosome damage by flow cytometry. I don't know how rare > chromosome > damage would be. Its not very hard to prepare chomsome preps from rats, either > con > A stimulated blood or perhaps you could look at granukoma pouch assay cells. > Just > a thought. We made some chromosome paints for rat and tried to look for > abnormal > chomosomes by microscopy also. > Simon > FLOWers > > I am in need of some help. > > We want to look at the protective effect of drug X on radiation induced > damage in the rat, by Flow Cytometry. > Does anyone have any thoughts?? > > I know this is potentially a very broad subject, but I would appreciate any > thoughts on potential avenues of approach > > thank you for your time and help > > Philip Barren > >
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