RE: cytoplasmic CD22

From: Hodge, Greg (HAEM) (hodgeg@mail.wch.sa.gov.au)
Date: Mon Sep 10 2001 - 17:22:51 EST

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> ----------
> From:		qli@sbmflab.org
> Sent:		Monday, 10 September 2001 23:02
> To:	Cytometry Mailing List
> Subject:	cytoplasmic CD22
>
> Quing,
>
> An easy solution to surface and I/C staining is to first stain surface
> with say CD22 FITC, FIX &PERM, then stain with CD22 PE. I would add CD19
> PC5 or similar for B cell confirmation and appropriate controls.
>
> Greg Hodge
> ----------------------------------------------------------------
> Hi, Colleagues,
>
> I need some help.  I am trying to determine the cytoplamic CD22 in
> B-Lymphocytes using Caltag's FIX &PERM reagent.  Since CD22 also expresses
> on the surface of B-Lymphocytes, how to find a method which only measures
> cytoplasmic not surface CD22? Also, how can I confirm that I am measuring
> cytoplasmic CD22?  Thanks.
>
> Qing Li, Ph.D.
> NTTD Department
> South Bend Medical Foundation
> South Bend, IN
>
>
>
>
>
> ----------
>

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