Re: Beads and apoptosis

From: Karim Vermaelen (Karim.Vermaelen@rug.ac.be)
Date: Wed Aug 29 2001 - 02:03:19 EST

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Check out: Eur. J. Immunol. 2000. 30: 2612?2619: It seems that in murine
dendritic cells, MHCII cross-linking initiates apoptosis of the APC, a
phenomenon counteracted by CD40 ligation. Maybe this is what you observe
with the anti-HLADQ beads on the B cells? Also (you will probably have done
it, but it's worthwhile mentioning) it's important to wash out the azide
from the beads.

"Acquarola, Nick" wrote:

> Does anybody know whether the HLA-DQ molecule has any association with
> apoptosis?
> We use the Dynal Dynabeads to isolate B cells for microlymphocytoxicity
> reactions and have noticed an abnormally higher (dead cell) background.
> One thought put to me was that the beads attached to the DQ molecule may
> initiate apoptosis.
>
> Any thoughts?
>
> Nick Acquarola

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