From: Simon Monard (smonard@trudeauinstitute.org)
Date: Mon Mar 12 2001 - 16:18:21 EST
Hi
People always used to incubate on ice for 30-60 minutes to try to prevent capping, also
to create a convenient time to have lunch or extended coffee and smoking breaks. Nowdays
you can add a tad of Sodium azide to prevent capping.
Simon
Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983
Ph 518 891 3080 X352
>>> "Heather Medbury" <Heather_Medbury@wsahs.nsw.gov.au> - 3/11/2001 5:50 PM >>>
Hi FLOWers,
About 5 years ago when I was analysing lymphocytes, we used to have the cell preparations
at 4 C for labelling with the antibody.
The work I do now on whole blood, the cells are labelled at room temperature.
Why do people label at 4 C when it works perfectly well at room temp?
Heather
Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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All experiments are preliminary
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"In his heart a man plans his course
but the Lord determines his steps"
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