Re: BONE MARROW LYSIS

From: Bgreig2@AOL.COM
Date: Tue Feb 20 2001 - 17:08:44 EST

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We currently use an Ammonium Chloride lysing procedure for both blood and bone marrow
and have good success maintaining high viability, good population capture, and  more
than adequate rbc disruption.  Attached is our procedure.

Good luck,
Bruce




Bruce Greig, Supervisor
Immunopathology Laboratory
Vanderbilt Univ. Med. Center
Nashville, TN. 37232

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