BrdU and To-Pro-3 iodide

From: Mark Coles (mcoles@nimr.mrc.ac.uk)
Date: Fri Feb 16 2001 - 20:22:30 EST

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Dear All,
	I recently obtained a flyer from Molecular Probes claiming
that To-Pro-3 iodide preferentially incorporated into BrdU labeled
DNA.  Thinking that it would be a good shortcut I have tried the dye
out to no success.  I have been staining and fixing mouse thymocytes
labeled with BrdU in 1% PF.  And then incubating the cells in the
presence of To-pro-iodide for 20min on ice and then running the
samples on a FACScalibur using the HeNe laser.	The To-Pro-3 iodide
definetly binds to the DNA, but I see no difference between cells
that have incorporated BrdU and those that have not.  Any suggestions.

Thanks Mark

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