Calcium flux experiments with standard BD Facscan/Facscalibur (instrument filters)?

Dear Flowers,

It's the holidays, and I have a hard time tracking down if I can do calcium
flux experiments (using Indo-1 AM or alike) by flow using the standard
equipped BD Facscan or Facscalibur instruments (in particular: filters ok?).

As I have only performed steady state experiments, I am also not sure if I
have to change the acquisition mode in order to allow analysis using time
as a parameter (either in CellQuest or FlowJo (to which we are slowly
transitioning)).

Any suggestions, hints, info, recommendations for data analysis?

Thank you all in advance for your help.

Ursula Esser

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