CD45 expression on ADP-activated murine platelets

Hello to all,

in recent experiments in two different mice strains we were analyzing the induction of
platelet-leukocyte aggregates as a function of platelet activation  based on staining
with CD41 and CD45. Interestingly, a strong rise in binding of CD45-PE/Cy5 was associated
with platelet activation independent of the low number of platelet-leukocyte aggregates
although CD45 at least in human is considered not to be expressed in platelets.

Looking through the literature, a cross-reactivity of CD45RA-antibodies with an
intracellular antigen in murine platelets has been published. My question, therefore,
is whether anyone observed a similar reactivity of CD45 clones with murine platelets
or can provide addtional data proving real expression or an artefact.

Regards,

Gregor Rothe