Keepings cells suspended in 1 to 5 % BSA/media helps prevent clumping. On Mon, 23 Oct 2000, Jeff Barry wrote: > > Dear Flow People, > > A colleague is trying to look for apoptosis using the sub G1, Propidium > Iodide, method however, his cells stubbornly refuses to form single cells > and continue to aggregate causing our FacsCalibur to block every time we put > the samples on. Can anyone relieve our misery by suggesting any sample > preparation tips that will prevent cell clumping. > > Regards, > > Jeff Barry > Paterson Institute > Manchester > UK > >Received on Tue Oct 24 13:50:13 2000
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