Try saponin. We add 10µl of a 3% soluiton to approximately 100µl of cells, wait a few minutes and then add our intracellular staining reagents. It does not appear to work for nuclear antigens. Randy Fischer NIH/NIAMS Building 9 Bethesda, MD 20892 fischer1@mail.nih.gov (301) 594-3537 > ---------- > From: KAUR, INDRESHPAL (JSC-SD) > Sent: Wednesday, September 6, 2000 9:37 AM > To: Cytometry Mailing List > > > Hello > Does any one know of a reagent that will permeabilize the cell membrane > and > not the nuclear membrane. BD's FACS lyse and pharmingens cytofix-cytoperm > will permeabilize both. > > Indresh Kaur Ph.D. > Phone: 281-483-8791 > > >Received on Thu Sep 7 10:39:10 2000
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